Application
This Anti-dimethyl Histone H3 (Arg26) antibody, Asymmetric, Trial Size is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H3 (Arg26).
Research Sub CategoryHistones
Research CategoryEpigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected dimethyl Histone H3 (Arg26), Asymmetric in A431 and HepG2 cells.
Dot Blot (Specificity) Analysis: 0.5 µg/mL from a representative lot detected dimethyl Histone H3 (Arg26), Asymmetric in Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
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Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Immunogen
KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3, asymmetrically dimethylated at Arg26.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality
Evaluated by Western Blotting in untreated and CARM1 treated HeLa acid extracts.
Western Blotting Analysis: 1 µg/mL of this antibody detects dimethyl Histone H3 (Arg26), Asymmetric in 10 µg of CARM1 treated HeLa acid extract.
Specificity
Broad species reactivity is expected based on immunogen sequence similarities.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~17 kDa observed
This product has met the following criteria: