ABE2879 Display Image

Anti-Dyskerin (Sheep)

Code: ABE2879 D2-231

Application

Western Blotting Analysis: A representative lot detected Dyskerin in 293T cells. The signal was depleted upon treatment with siRNA against Dyskerin (Stern, J.L., ...


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Application

Western Blotting Analysis: A representative lot detected Dyskerin in 293T cells. The signal was depleted upon treatment with siRNA against Dyskerin (Stern, J.L., et. al. (2012). Mol Cell Biol. 32(13):2384-95).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Dyskerin associated to telomeres in telomerase-overexpressing HEK293 cells (Stern, J.L., et. al. (2012). Mol Cell Biol. 32(13):2384-95).

Immunoprecipitation Analysis: A representative lot recoverd Dyskerin telomerase-overexpressing 293T cells. (Stern, J.L., et. al. (2012). Mol Cell Biol. 32(13):2384-95).

Western Blotting Analysis: A representative lot detected Dyskerin in HEK293T cells transfected with a dyskerin expression plasmid (Courtesy of the laboratories of Dr. Hilda Pickett and Dr. Tracy Bryan, Children s Medical Research Institute, Australia).

Research CategoryEpigenetics & Nuclear Function

Anti-Dyskerin (Sheep), Cat. No. ABE2879, is a highly specific sheep polyclonal antibody that targets H/ACA ribonucleoprotein complex subunit 4 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

H/ACA ribonucleoprotein complex subunit 4 (UniProt: O60832; also known as CBF5 homolog, Dyskerin, Nopp140-associated protein of 57 kDa, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1) is encoded by the DKC1 (also known as NOLA4) gene (Gene ID: 1736) in human. Dyskerin is a highly conserved nucleolar protein that is involved with multiple cellular functions, such as protein expression, growth, proliferation, and nucleo-cytoplasmic shuttling. It helps in the stabilization of the telomerase RNA component, which allows for the proper functioning of telomerase enzymatic complex. It also modifies specific uridine residues of ribosomal RNA by converting them to pseudouridine, thus allowing proper ribosome processing and function. Point mutations in the DKC1 gene result in a rare inherited syndrome called X-linked dyskeratosis congenita, which is leads to early mortality due to progressive bone marrow failure and is characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. (Ref.: Montanaro, L (2010). J. Pathol. 222(4): 345-9).

Immunogen

Diphtheria Toxoid Carrier Protein-conugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Dyskerin.

Epitope: C-terminal half

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity Purified

Purified sheep polyclonal antibody in buffer containing 20 mM HEPES-KOH (pH 7.9) 200 mM KCl 1 mM EDTA with 0.02% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell Lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Dyskerin in HeLa cell Lysate.

Specificity

This sheep polyclonal antibody detects Dyskerin in human cells. It targets a 16 amino acid epitope within the C-terminal half of human Dyskerin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~58 kDa observed; 57.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcesheep
clonepolyclonal
Gene Informationhuman ... DKC1(1736)
isotypeIgG
NCBI accession no.NP_001354
packagingantibody small pack of 25 µg
purified byaffinity chromatography
Quality Level100
species reactivityhuman
technique(s)western blot: suitable, ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable
UniProt accession no.O60832
Code
Description
Unit Size
List Price
Qty
ABE2879-25UG
Unit:25UG
List Price: €155.00
Source:List Price
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