Analysis Note

ControlA431 cell lysate

Application

Anti-Tristetraprolin Antibody is an antibody against Tristetraprolin for use in Western Blotting, ICC, Immunoprecipitation, RNA Binding Protein Immunoprecipitation (RIP).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory to detect Tristetraprolin in human leukocytes. (Brooks, S., et al. (2002). Arthritis and Rheumatism. 46(5):1362–1370.)

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tristetraprolin (TTP, Nup 475, TIS11, G0S24, or ZFP36) is a member of the CCCH tandem zinc finger protein family. It is a nuclear RNA-binding protein that functions as a trans-acting factor. It is involved in post-transcriptional regulation of cytokine genes, most notably Tumor Necrosis Factor alpha (TNF-α). Upon activation by lipopolysaccharides, TTP binds TNF-α mRNA at AU-rich domains in the 3′-untranslated Region (3′-UTR) and induces deadenylation, thereby destabilizing the mRNA and tagging it for degradation. To produce this effect, TTP recruits other trans-acting factors to its C-terminal domain and mRNA-decay enzymes to its N-terminal domain. TTP is deactivated by phosphorylation driven by mitogen-activated protein kinase-activated protein kinase 2 (MK2) and p53. Therefore, TTP/MK2 regulates the biosynthesis of TNF-α and mediates inflammatory responses involving TNF-α. Knockout of TTP in mice produces arthritis, alopecia, dermatitis, among a range of other phenotypes related to overexpression of TNF-α.

Immunogen

KLH-conjugated linear peptide corresponding to human Tristetraprolin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing PBS without 0.05% sodium azide.

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Tristetraprolin on 10 µg of A431 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~40 kDa observed. Uniprot gives a calculated molecular weight of 34 kDa An uncharacterized band appears at ~53 kDa in some lysates.