Analysis Note

ControlMouse embryonic stem cell lysate

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZH1 in human lung and kidney tissues.

Research CategoryEpigenetics & Nuclear Function

Detect the EZH1 protein using this Anti-EZH1 validated for use in IHC & WB.

Research Sub CategoryHistone Modifying Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH1 is the catalytic subunit of the PRC2/EED-EZH1 complex. This complex methylates Lys-27 of histone H3, which leads to transcriptional repression of the affected target gene. EZH1 is required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in maintaining the identity of embryonic stem cells.

Immunogen

KLH-conjugated linear peptide corresponding to human EZH1.

Linkage

Replaces: 04-1047

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected EZH1 in 10 µg of mouse embryonic stem cell lysate.

Specificity

This antibody potentially recognizes all isoforms of EZH1.

Other homologies: Porcine (94% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~85 kDa observed. Uniprot states 5 isoforms for EZH1. This antibody potentially recognizes all isoforms. Isoform A is 85,271 Daltons. Isoform B is 86,025 Daltons. Isoform C is 80,699 Daltons. Isoform D is 76,952 Daltons. Isoform E is 68,961 Daltons. (Margueron, 2008).