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Anti-monomethyl-Histone H3 (Lys36)

Code: ABE244 D2-231

Analysis Note

ControlHeLa acid extract and various recombinant Histone proteins

Application

Research Sub CategoryHistones

Research Category...


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Analysis Note

ControlHeLa acid extract and various recombinant Histone proteins

Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Anti-monomethyl-Histone H3 (Lys36) Antibody is a rabbit polyclonal antibody for detection of Histone H3 monomethylated at lysine 36. This purified polyclonal antibody also known as Anti-H3K36me1, has dot blot (DB) proven specificity & has been validated in WB, IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl-Histone H3 (Lys36) from HeLa acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see table) were probed with Anti-monomethyl-Histone H3 (Lys36) (0.5 µg/mL).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of four histones that form a core octameric structure that is required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can alter chromatin structure and stability, and influence DNA repair and gene expression. Histone H3 undergoes methylation of its lysine and arginine residues, with varying biological outcomes. The lysine 36 residue undergoes mono-, di-, or trimethylation which may be performed by one of many methyltransferase enzymes (SET2, NSD1, NSD2, NSD3, ASH1, SMYD2, SETMAR, KMT3A, and SETD3). Several studies have indicated that methylated lysine 36 residues promote Rpd3S-mediated deacetylation of other histones resulting in gene activation.

Immunogen

Epitope: Monomethylated Lys36

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3 monomethylated at Lys36.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

Western Blot Analysis: 0.05 µg/mL of this antibody detected monomethyl-Histone H3 (Lys36) in 10 µg of HeLa acid extract.

Specificity

This antibody recognizes the N-terminus of Histone H3 monomethylated at Lys36.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~17 kDa observed

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... HIST1H3F(8968)
NCBI accession no.NP_003484
purified byaffinity chromatography
Quality Level100
shipped inwet ice
species reactivityrat, mouse, human
technique(s)western blot: suitable, immunoprecipitation (IP): suitable, dot blot: suitable
UniProt accession no.Q16695
This product has met the following criteria: