Anti-CUX1; a.a. 861

Analysis Note

ControlHeLa nuclear extract

Application

Research Sub CategoryTranscription Factors

Immunoprecipitation Analysis: 1.25 µg from a representative lot immunoprecipitated CUX1 in 500 µg of HeLa nuclear extract.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Research CategoryEpigenetics & Nuclear Function

Detect Cux1 using this rabbit polyclonal antibody, Anti-CUX1 Antibody, a.a. 861 validated for use in western blotting & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Homeobox protein cut-like 1 (CUX1) is also known as CCAAT displacement protein (CDP) and Homeobox protein cux-1. CUX1 is thought to repress expression of developmentally regulated genes involved in mammalian development, likely through the prevention of CCAAT factor binding at promoters. CUX1 also represses T-cell receptor (TCR) beta enhancer function by binding to matrix attachment region beta (MARbeta), and is a component of the nf-munr repressor, which binds to matrix attachment regions (MARs) of the immunoglobulin heavy chain enhancer.

Immunogen

GST-tagged recombinant protein corresponding to a region of amino acids beginning at 861 of human CUX1.

Epitope: Region of amino acids beginning at 861

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CUX1 in 10 µg of HeLa nuclear extract.

Specificity

Other homologies: Mouse (95% sequence homology).

This antibody recognizes the region of amino acids beginning at 861 of CUX1. This antibody is expected to cross react with all known isoforms of CUX1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~200 kDa and ~110 kDa observed. Uniprot describes 11 different isoforms. A full length protein has been described at 200 kDa and is proteolytically cleaved into the p150, p110, p90, and p80 isoforms. Additionally, the p75 isoform is the result of alternative splicing (Sansregret, L, et al. (2008). Gene. 412(1-2):84-94.). Uncharacterized band(s) may be observed in some cell lysates.