Application

Research Sub CategoryHistones

Detect MLL2 (C-Term) using this Anti-MLL2 antibody (C-Term) validated for use in western blotting & IHC.

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected MLL2 (C-term) in human breast cancer tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MLL2, also known as Histone-lysine N-methyltransferase 2D, or Lysine N-methyltransferase 2D or ALL1-related protein, or Myeloid/lymphoid or mixed-lineage leukemia protein 2, and encoded by the gene KMT2D/ALR/MLL2/MLL4, is a histone methyltransferase that methylates Lys-4 of histone H3 to form H3K4me. H3K4me represents a specific tag for epigenetic transcriptional activation and for instance in estrogen receptor function where MLL2 acts as coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription. MLL2 is also a component of the ASCOM/Mll2/3 complex which plays an important role during development particularly in lymphomagenesis. MLL2 is localized to the nucleus. MLL2 is expressed in most embryonic and adult tissues. Defects in MLL2 are associated with Kabuki syndrome, a congenital mental retardation syndrome with additional features, including postnatal dwarfism, facial and bone deformities. Defects are also associated with medulloblastoma a highly malignant primary brain tumor.

Immunogen

GST-tagged recombinant protein corresponding to the C-terminus of human MLL2 (C-term).

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MLL2 (C-term) in 10 µg of human testis tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed