Application

Research Sub CategoryHistones

Anti-Ash1L Antibody detects level of Ash1L & has been published & validated for use in Ash1L.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ash1L, also known as Histone-lysine N-methyltransferase ASH1L, or ASH1-like protein (huASH1), Absent small and homeotic disks protein 1 homolog, or Lysine N-methyltransferase 2H, and encoded by the gene ASH1L/KIAA1420/KMT2H, is a histone methyltransferase that specifically methylates Lys-36 of histone H3 (H3K36me). ASH1L is one of several H3K36-specific methyltransferases. Others include HYPB, NSD1 and NSD2 proteins. ASH1L′s activity is regulated by many processes but one interesting one is the inhibition of ASH1L by the presence of ubiquitinated histone H2a (ubH2a). All of the H3K36-specific methyltransferases, including ASH1L, HYPB, NSD1 and NSD2 appear to be inhibited by ubH2A, whereas the other histone methyltransferases, including PRC2, G9a and Pr-Set7 are not affected by ubH2A. This finding helps to partially explain how and why particular histone H3 proteins get methylated while others do not and thus provide some insight into is the mechanisms involved in polycomb chromatin structure and its effects on gene expression or silencing. Ash1L is localized to the nucleus and tight junctions and is widely expressed.

Immunogen

KLH-conjugated linear peptide corresponding to human Ash1L.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ash1L in 10 µg of HeLa nuclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~330 kDa observed, Ash1L degradation product may be observed at ~150 kDa band. Uncharacterized band(s) may appear in some lysates.