Application

Immunoprecipitation Analysis: A representative lot detected Exo1 in HeLa cell lysate (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Exo1 in HeLa and siExo1 hela cell lysates (Courtesy of Dr. Zhongsheng You at Washington University in St. Louis).

Research CategoryEpigenetics & Nuclear Function

Anti-Exo1, Cat. No. ABE1354, is a highly specific rabbit polyclonal antibody that targets Exonuclease 1 and has been tested for use in Immunoprecipitation and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Exonuclease 1 (UniProt: Q9UQ84; also known as hExo1, Exonuclease I, hExoI) is encoded by the EXO1 (also known as EXOI, HEX1) gene (Gene ID: 9156) in human. Exonuclease 1 is a magnesium-dependent protein with 5′ to 3′ exonuclease and an RNase H activity. It may also possess a cryptic 3′→5′ double-stranded DNA exonuclease activity. It is highly expressed in bone marrow, testis and thymus. Higher levels of expression are also observed in fetal liver. Lower expression has been observed in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen, and stomach. It functions in DNA mismatch repair to excise mismatch-containing DNA tracts directed by strand breaks located either 5′ or 3′ to the mismatch. It interacts physically with the DNA mismatch repair proteins, Msh2 and Mlh1, and is involved in the excision of the mispaired nucleotide. Exonuclease 1 is phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. It is shown to be phosphorylated at Serine 454, Threonine 621, and Serine 714 upon DNA-damage caused by treatment with hydroxyurea, but not upon IR treatment. The hydroxyurea-induced triple phosphorylation of exonuclease 1 facilitates destabilization/degradation of the protein.

Immunogen

KLH-conjugted linear peptide corresponding to 16 amino acids from the C-terminal half of human Exonuclease 1. The immunogen sequence is conserved in both isoforms 1 and 2.

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in 293T cells transfected with GFP-Exo1.

Western Blotting Analysis: 2 µg/mL of this antibody detected Exo1 in 293T cells transfected with GFP-Exo1.

Specificity

This rabbit polyclonal antibody detects Exonuclease 1 in human cells. It targets an epitope within 16 amino acids from the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~145 kDa observed; 94.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).