Anti-Histone H2A.Z (C-term)

Application

This Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1µg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.

ChIP-seq: A representative lot of this antibody (0.5 µg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.

Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.

Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.

General description

H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

Immunogen

KLH-conjugated linear peptide corresponding to region the near C-terminus of Human Histone H2A.Z.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.

Quality

Evaluated by Western Blotting using recombinant histones

Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.

Specificity

Recognizes histone H2A.Z

wide range expected

Target description

~16 kDa observed