Anti-phospho Carm1 (Ser228)

Analysis Note

ControlEGF treated and untreated A431 cell lysates

Application

Research CategoryEpigenetics & Nuclear Function

Anti-phospho CARM1 (Ser228) Antibody is a Rabbit Polyclonal Antibody for detection of phospho CARM1 (Ser228) also known as Protein arginine N-methyltransferase 4 & has been validated in WB.

Research Sub CategoryChromatin BiologyHistones

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CARM1 (coactivator-associated arginine methyltransferase 1) is an arginine N-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to arginine residues. This protein-arginine methyltransferase that works in conjunction with p300 and GRIP1 to enhance transcription. CARM1 methylation targets include histone H3 at Arg17 and p300 at Arg2142. CARM1 is recruited to promoters upon gene activation together with histone acetyltransferases from the p300 and p160 families. During monocyte inflammatory stimulation, CARM1 acts together with p300 as a coactivator for NF-kB. It is responsible for moving cells toward the inner cell mass in developing blastocysts. CARM1 also plays an important role in androgen receptors and may play a role in prostate cancer progression.

Immunogen

Epitope: Phosphorylated Ser228

KLH-conjugated linear peptide corresponding to human CARM1 phosphorylated at Ser228.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in EGF treated and untreated A431 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected CARM1 on 10 µg of EGF treated and untreated A431 cell lysates.

Specificity

This antibody recognizes CARM1 phosphorylated at Ser228.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~63 kDa observed