Analysis Note

ControlRat brain tissue lysate

Application

Research CategoryStem Cell Research

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PRDM16 in rat brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected PRDM16 in human brain cells.

Detect PRDM16 using this rabbit polyclonal antibody, Anti-PRDM16 Antibody validated for use in western blotting, IHC & Immunofluorescence.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PR domain zinc finger protein 16 (PRDM16) is also called Transcription factor MEL1 (MDS1/EVI1-like gene 1). PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. PRDM16 is expressed in the uterus and kidney. PRDM16 regulates transcription through DNA binding and plays a role in the differentiation of myoblastic precursors into brown adipose tissue (BAT), and represses TGF-beta signalling. Translocations in PRDM16 occur in subsets of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) cases.

Immunogen

KLH-conjugated linear peptide corresponding to human PRDM16.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PRDM16 in 15 µg of rat brain tissue lysate.

Specificity

This antibody can recognize all four isoforms of PRDM16.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~100 kDa, ~135 kDa and ~150 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 4 isoforms between ~120 kDa and ~140 kDa