Application

This Anti-RINT-1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of RINT-1.

Research CategoryApoptosis & Cancer

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected RINT-1 in A431, HeLa, HepG2, and NIH/3T3 cells.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RAD50-interacting protein 1 (UniProt Q6NUQ1; also known as HsRINT-1, RINT-1, RAD50 interactor 1) is encoded by the RINT1 gene (Gene ID 60561) in human. Originally identified and named as a RAD50-interacting partner by a yeast two-hybrid screening, RINT1 is involved in the G2/M checkpoint, maintenance of centrosome integrity, telomere elongation, Golgi apparatus, Golgi-ER vesicular trafficking and Cis- and Trans-Golgi homeostasis. Rint1 homozygous deletion in mice causes early embryonic lethality, while Rint1 heterozygotes are predisposed to multiple tumor formation. Likewise, rare RINT1 mutations are identified to be associated with intermediate levels of breast cancer risk in human.

Immunogen

KLH-conjugated linear peptide corresponding to an N-terminal sequence of human RINT-1.

Epitope: N-terminal region.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in MOLT-4 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RINT-1 in 10 µg of MOLT-4 cell lysate.

Specificity

This polyclonal antibody detects RINT-1 by targeting an N-terminal region sequence.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~85 kDa observed. 90.63 kDa calculated. Target band may be resolved into a doublet with the slower migrating band representing product resulting from translation starting at an non-AUG codon (Xiao, J., et al. (2001). J. Biol. Chem. 276(9):6105-6111). Uncharacterized band(s) may appear in some lysates.