Anti-MACC1

Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected MACC1 in human liver tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected MACC1 in human liver cells.

Anti-MACC1 Antibody is an antibody against MACC1 for use in western blotting, IHC & immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Metastasis-associated in colon cancer protein 1, also known as SH3 domain-containing protein 7a5, and encoded by the gene name MACC1, is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (MET) pathway. This pathway is involved in cellular growth, epithelialmesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 was found significantly upregulated in malignant tissues (colon cancer of all stages as well as liver and lung metastases) compared to normal tissues or adenomas. MACC1 represents an early and crucial prognostic indicator for colon cancer metastasis that is independent of age, sex, tumor infiltration, nodal status, and lymph vessel invasion. Besides its involvement in signal transduction with the MET receptor, MACC1 also links MET signaling and apoptosis. MACC1 may also be an important therapeutic target for colorectal cancer treatment.

Immunogen

KLH-conjugated synthetic peptide corresponding to the center region of human MACC1.

Epitope: center region

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in Mouse liver tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MACC1 in Mouse liver tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~93 kDa observed. Uncharacterized bands may be observed in some lysate(s).