Application

Research CategoryApoptosis & Cancer

Anti-Cathepsin S. Cat. No. ABC503, is a rabbit polyclonal antibody that detects Cathepsin S and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cathepsin S in human lymphoma, human small intestine, and human pancreas tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cathepsin S (Uniprot: P25774; EC: 3.4.22.27) is encoded by the CTSS gene (Gene ID: 1520) in human. Cathepsin S is a lysosomal thiol protease that removes invariant chain from MHC class II molecules. It displays higher activity on ZVal-val-Arg-/-Xaa compounds and less activity on Z-Phe-Arg-/-NHMec. Cathepsin S is secreted with a signal (aa 1-16) and a pro-peptide (aa 17-114) sequence that are cleaved to produce active enzyme (aa 115-331). Cathepsin S is involved in antigen presentation, general protein catabolism, and tumor progression. Because of its redistribution or increased levels in human and animal tumors, cathepsin S plays a role in invasion and metastasis. Inhibition of cathepsin S is shown to induce autophagy in tumor cells through EGFR signaling pathway by facilitating EGF-mediated EGFR degradation. Cathepsin S inhibition is shown to delay EGFR degradation and cause EGFR accumulation in the late endosomes at the perinuclear region. (Ref.: Beers, C et al. (2005). J. Immunol. 174(3): 1205-1212; Huang CC et al. (2016). Sci. Rep. 6:29256).

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human cathepsin S.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Cathepsin S in 10 µg of human testis tissue lysate.

Specificity

This polyclonal antibody detects cathepsin S in human cells. It targets an epitope within 21 amino acids from the internal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed; 37.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).