Anti-ATG13

Analysis Note

ControlRat heart tissue lysate

Application

Anti-ATG13 Antibody is a highly specific rabbit polyclonal antibody, that targets ATG13 & has been tested in western blotting, IHC & Immunofluorescence.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected ATG13 in mouse heart tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected ATG13 in mouse heart cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Autophagy-related protein 13 (ATG13) is an autophagy factor that is involved in the preservation of cellular nutrients under starvation conditions as well as under normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. Knockdown of ATG13 inhibits autophagosome formation.

Immunogen

KLH-conjugated linear peptide corresponding to human ATG13.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in rat heart tissue lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1 µg/mL of this antibody detected ATG13 in 15 µg of rat heart tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~49 kDa observed. Uniprot describes 5 isoforms ranging between ~44 kDa and ~60 kDa Uncharacterized bands may appear in some lysate(s).