Analysis Note

ControlHepG2 cell lysate

Application

Research CategoryInflammation & Immunology

This Anti-PEX13 (peroxisomal membrane marker) Antibody is validated for use in Western Blotting, ICC for the detection of PEX13 (peroxisomal membrane marker).

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Peroxisomal membrane protein 13 (PEX13) is a component of the peroxisomal translocation machinery, which binds to peroxisomal targeting signal receptors via its SH3 domain, and facilitates the import of these receptors into the peroxisome membrane. Defects in PEX13 have been linked to neurodegenerative disorders, such as Zellweger syndrome.

Immunogen

Recombinant protein corresponding to human PEX13 (peroxisomal membrane marker 13).

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected PEX13 (peroxisomal membrane marker) in HepG2 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~49 kDa observed. This protein has an expected molecular weight of 44 kDa; however, it has been observed at ~49 kDa (Muller, C. C., et al. 2011. Dis Model Mech. 4(1):104-110.). An uncharacterized band may be observed at ~41 kDa in some cell lysates.