Analysis Note

ControlT47D cell lysate

Application

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected DHODH in human testis and breast carcinoma sections.

Anti-DHODH Antibody is a rabbit polyclonal antibody, validated for use in western blotting & IHC (Paraffin).

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dihydroorotate oxidase (DHODH) is also called Dihydroorotate dehydrogenase (quinone), mitochondrial (DHOdehase). DHODH is a member the dihydroorotate dehydrogenase (Type 2) family and is involved in pyrimidine biosynthesis. DHODH catalyzes the conversion of dihydroorotate to orotate using quinone as an electron acceptor. DHODH defects can cause postaxial acrofacial dysostosis (POADS).

Immunogen

KLH-conjugated linear peptide corresponding to the Topological Domain (Mitochondrial intermembrane) of human DHODH.

Epitope: Topological Domain ( Mitochondrial intermembrane)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected DHODH in 10 µg of T47D cell lysate.

Specificity

Other Homologies: Rat (93% sequence homology) and Mouse (86% sequence homology).

This antibody recognizes the Topological Domain (Mitochondrial intermembrane) of DHODH.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~43 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.