Anti-NMDAR1 Rabbit Monoclonal

Analysis Note

ControlRat brain tissue lysate

Application

Anti-NMDAR1 Antibody, clone 1.17.2.6 detects level of NMDAR1 & has been published & validated for use in WB, IH.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Western Blot Analysis: 0.1 µg/mL of this antibody from a previous lot detected NMDAR1 on 10 µg of mouse microsomal brain tissue lysate.

Immunohistochemistry Analysis: 1:250 dilution of this antibody from a previous lot detected NMDAR1 in rat cerebellum tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) is a multi-pass membrane protein belonging to the glutamate-gated ion channel family and is one of two subunits comprising the tetrameric protein NMDAR localized throughout the brain at excitatory synapses. NMDAR1 is mediated by glycine and is a critical component to excitotoxicity, learning, memory acquisition, synaptic plasticity, and synaptogenesis. It also plays a regulatory role for neuronal functions involving glutamate neurotransmission and the targeting of NMDA receptors on the cell surface. As a glutamate-gated ion channel, NMDAR1 is highly calcium permeable and possesses sensitivity to magnesium in a voltage-dependent manner.

Immunogen

Linear peptide corresponding to human NMDAR1.

Epitope: Recognizes NMDAR1 at the C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 40% Glycerol.

Protein A

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected NMDAR1 on 10 µg of rat brain tissue lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~120 kDa observed