NMDAR1; RBX-50UL

Analysis Note

ControlBrain tissue

Application

This Anti-NMDAR1 Antibody is validated for use in IH, WB for the detection of NMDAR1.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Immunohistochemistry:
AB9864 can be used for using 4% paraformaldehyde or paraformaldehyde/glutaraldehyde fixed rat tissue. Suggested working dilution for light microscopy is 1:1,000-1:2,000.

Western blot:
1:1,000-1:2,000 using ECL on rat brain lysate.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-methyl-D-aspartate Receptors (NMDAR) are one of three pharmacologically distinct subtypes of ionotropic receptors that mediate a majority of excitatory neurotransmission in the brain via the endogenous amino acid, L-glutamate. MDARs form heteromeric complexes usually composed of two components, the NR1 subunit and NR2 subunits. Both proteins are weakly expressed throughout the brain but show localization in the dendritic region of the mushroom bodies. The NMDAR channel is highly permeable to Ca2+ and Na+ and its opening requires the simultaneous binding of glutamate and postsynaptic membrane depolarization. The activated NMDAR channel allows calcium influx into the post-synaptic cell where calcium triggers a cascade of biochemical events that create synaptic changes. NMDAR have been studied extensively in Drosophila for its potential relationship to behavioral plasticity. A newly identified NMDAR complex of more than 80 different proteins has been identified. Genetic and pharmacological disruptions of components of the NMDAR complex produce learning impairments in rodents, further linking behavioral plasticity to synaptic plasticity.

Immunogen

Epitope: a.a. 909-938

Synthetic peptide corresponding to the C-terminus of rat NMDA receptor subunit (Catalog number AG344). {amino acids 909-938 rat NMDAR1{LQNQKDTVLPRRAIERE EGQLQLCSRHRES}

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1516; 07-362

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit monoclonal supernatant liquid containing 0.05% sodium azide.

Unpurified

Quality

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected NMDAR1 on 10 µg of rat brain lysates

Specificity

The original sequence used is selective for splice variants NR1-1a, NR1-1b, NR1-2a, NR1-2b using the nomenclature defined by Hollmann, M. et al. (1993). These appear to be the major splice variants expressed in rat brain (Sugihara H, et al., 1992). No cross-reaction with other glutamate receptor subunits. Western Blot analysis shows that this rabbit monoclonal antibody labels a single band at approximately 120 kD corresponding to NMDAR1 in rat brain lysate.

Reactivity in mouse is weak. On reduced Western blots a band matching that in rat is observed but tests in immunohistochemistry have been unsuccessful. It is expected that the antibody will also react with monkey, gerbil, rabbit, feline and fish based on the fact that another antibody made to the same peptide sequence reacts with those species.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

120 kDa