Anti-Pancreatic Polypeptide

Application

Research CategoryNeuroscience

Western Blotting Analysis: 1:2,000 dilution from a representative lot detected Pancreatic Polypeptide in 10 µg of human pancreas tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Pancreatic Polypeptide in human pancreas tissue sections.

Immunocytochemistry Analysis (ICC): A 1:500 dilution from a representative lot detected Pancreatic Polypeptide in TGP52 cell line.

Anti-Pancreatic Polypeptide Antibody, Cat. No. AB939-I, is a highly specific rabbit polyclonal antibody that targets Pancreatic polypeptide and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pancreatic polypeptide (UniProt: P01298; also known as PP, Obinepitide, Pancreatic prohormone) is encoded by the PPY (also known as PNP) gene (Gene ID: 5539) in human. Pancreatic polypeptide is a member of the neuropeptide Y family and is secreted protein that is produced by PP cells in the pancreatic islets of Langerhans. It is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off. It acts as a regulator of pancreatic and gastrointestinal functions. Two isoforms of pancreatic polypeptide have been described that are generated by alternative splicing. Pancreatic polypeptide release is functionally associated with increased satiety, delayed gastric emptying, inhibition of gallbladder motility, and regulation of energy expenditure. Its deficiency is observed in cases of pancreatic resection and chronic pancreatitis and is associated with significant reduction in PP cell mass and abnormally high hepatic glucose production even in the presence of endogenous insulin. (Ref.: Maxwell, J et al. (2014). Pancreas 43(4); 651-656).

Immunogen

Epitope: cytoplasmic domain

KLH-conjugated linear peptide corresponding to 36 amino acids the internal region of human pancreatic polypeptide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: 1:2,000 dilution of this antibody detected Pancreatic Polypeptide in 10 µg of PANC-1 cell lysate.

Specificity

This rabbit polyclonal antibody detects both isoforms of human pancreatic polypeptide. It targets an epitope within 36 amino acids from the internal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~11 and ~20 kDa observed for isoforms 1 and 2; 10.45 and 18.79 kDa calculated for isoforms 1 and 2. Uncharacterized bands may be observed in some lysate(s).