Application

Immunohistochemistry: 1:500-1:1,000 (see application notes that follow).

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB9358

IMMUNOHISTOCHEMISTRY

Suggested perfusion: 1) Ca2+-free Tyrode′s solution followed by 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB9358. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with fluorescently labeled secondary antibody.

This Anti-Neuropeptide Y2 Receptor Antibody is validated for use in IH for the detection of Neuropeptide Y2 Receptor.

Research CategoryNeuroscience

Research Sub CategoryCNS Control of MetabolismHormones & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide from Neuropeptide Y2 Receptor.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.05% sodium azide.

Specificity

Recognizes Neuropeptide Y2 Receptor.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.