Application

Research Sub CategoryReward & AddictionSignaling Neuroscience

Detect DARPP-32 using this Anti-DARPP-32 Antibody, phosphoThr75 validated for use in WB.

Research CategoryNeuroscience

Western blot: 1:1,000. The antibody recognizes the ~34 kD DARPP-32 protein phosphorylated at Thr75 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: phosphoThr75

Synthetic peptide from human/mouse DARPP-32, phosphoThr75.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 µg per mL BSA with 50% glycerol.

Specificity

DARPP-32, phosphoThr75.

Storage and Stability

Maintain at -20°C to -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.