DARPP-32 PTHR34; RBX-100UL

Analysis Note

ControlCultured primary neurons, rat brain lysate and human breast adenocarcinoma

Application

Research Sub CategoryReward & AddictionSignaling Neuroscience

This Anti-DARPP-32 Antibody, phosphoThr34 is validated for use in WB for the detection of DARPP-32.

Research CategoryNeuroscience

Western blot: 1:1,000. The antibody recognizes the ~32 kD DARPP-32 protein phosphorylated at Thr34 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide from DARPP-32, phosphoThr34.

Epitope: phosphoThr34

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 µg per mL BSA with 50% glycerol.

ImmunoAffinity Purified

Specificity

Recognizes DARPP-32 phosphorylated at threonine 34 .

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

~32 kD