Analysis Note
ControlImmunocytochemistry positive control: U251 glioma cells; negative control MCF7 breast carcinoma.
Western Blot positive control: HT1080 fibrosarcoma, negative control: MCF7 breast carcinoma
Application
Research CategoryCell Structure
Research Sub CategoryMMPs & TIMPs
Immunocytochemistry: 5-10 µg / ml
Western blot: 5 µg / ml Expected band sizes: 60-63 kDA and 42 kDa pro-enzyme and degradation form respectively.
Immunopreciptation: 1-2 µg / mg total protein in 500 µl reaction volume.
FACS Analysis: 5 µg/ mL (1 million cells per mL)
EIA: 1:10,000
Optimal working dilutions must be determined by the end user.
Anti-MMP-14 Antibody detects level of MMP-14 & has been published & validated for use in ELISA, FC, IP, WB & IC.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Catalytic domain of MT1-MMP
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Liquid in PBS, pH 7.6, containing 0.01% sodium azide as a preservative.
Specificity
The antibody recognizes native and recombinant proteins.
Storage and Stability
Store antibody at -20°C for 12 months. Avoid repeat freeze thaw cycles.
This product has met the following criteria to qualify for the following awards: