COLLAGEN VI; RBX HU-500UL

Application

Anti-Collagen Type VI Antibody is an antibody against Collagen Type VI for use in ELISA, IH.

Immunohistochemistry: 1:10-1:40 dilution of reconstitute for indirect immunofluorescent staining of frozen tissue sections.

ELISA: 1:10,000 dilution of reconstitute for ELISA on human collagen type VI.

Optimal working dilutions must be determined by the end user.

Immunogen

Human placental collagen type VI

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Pooled antisera was cross-absorbed against immobilized human serum proteins and collagen types IV and V. The IgG fraction was then precipitated with ammonium sulfate and subsequently dialyzed. Lyophilized from 500µL of 10mM Na-phosphate, 150mM NaCl, pH 7.5 with 0.1% mannitol.

Specificity

Human collagen type I ﹤0.5%

Human collagen type II ﹤0.5%

Human collagen type III ﹤0.5%

Human collagen type IV ﹤0.5%

Human collagen type V 5%

Human collagen type VI 100%

Human fibronectin ﹤0.5%

Human albumin ﹤0.5%

Human immunoglobulin ﹤0.5%

Storage and Stability

Maintain lyophilized at -20°C for up to 12 months. Reconstitute with 500uL of distilled water or saline. Store reconstitute between 2 and 8°C for up to two months. Remove any insoluble material by microcentrifugation before use.