COLLAGEN II; RB X HU-500UL

Application

Research CategoryCell Structure

ELISA 1:1000.

Indirect immunofluorescence on cryostat sections or cultured cells 1:10-1:20

Western Blot 1:100

Optimal working dilutions must be determined by the end user.

Anti-Collagen Type II Antibody detects level of Collagen Type II & has been published & validated for use in ELISA, WB, IC, IH.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Collagen type II extracted and purified from human cartilage.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit antiserum passed over agarose sorbents with immobilized human blood plasma proteins and human collagen types I, III, IV and V. Antibody was affinity purified on immobilized human collagen type II.

Product is liquid in 0.15M sodium chloride, 10mM sodium phosphate pH 7.5 with 0.1% mannitol and 0.1% dextran as stabilizers. No preservative.

Specificity

The antibody reacts with both native and denatured human collagen type II.



Cross Reactivity Percent

Human collagen type II 90%

Human collagen type I, III, IV, V ﹤1%

Human plasma proteins do not interfere with binding to collagen.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of reciept. Remove any nondispersed aggregates by microcentrifugation prior to use.