Application

Research CategoryCell Structure

ELISA on human collagen type IV: 1:3,000

Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40, fresh frozen or acetone fixed specimens only.

Dot and slot blots: 1:300.

Not recommended for use in Western blot, or for use on paraffin-embedded tissue sections.

Optimal working dilutions must be determined by the end user.

Anti-Collagen Type IV Antibody detects level of Collagen Type IV & has been published & validated for use in ELISA, IH.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified human placental collagen type IV

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified antibody from cross-absorbed antiserum. Liquid in PBS containing mannitol, dextran and no preservatives.

Format: Purified

Specificity

Antibody reacts with native and heat denatured human type IV collagen. Less than 10% cross reactivity with collagen types I, III, and V, and less than 1% with human collagen II. Antiserum was passed over immobilized human blood plasma proteins, and human collagen types I, III, and V. Human plasma proteins do not interfere with binding to collagen.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze / thaw cycles.