Anti-Stabilin-1 (C-terminus)

Analysis Note

ControlHUVEC lysate.

Application

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Stabilin-1 in human placental tertiary villi.
Immunofluorescence Analysis: A previous lot of Anti-Stabilin-1, C-terminus was shown to positively stain using immunofluorescence techniques (Kzhyshkowska, 2008).

Anti-Stabilin-1 Antibody, C-terminus is an antibody against Stabilin-1 for use in WB, IH(P) & IF.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Stabilin-1 and Stabilin-2 constitute a novel family of fasciclin domain-containing hyaluronan receptor homologs that may play a role in cell-cell and cell-extracellular matrix interactions in vascular function and inflammatory processes. Stabilin-1 is a transmembrane protein, expressed in endothelial cells within the sinusoidal regions of liver, spleen, and lymph tissues as well as macrophages. Stabilin-2 is absent from the latter (Politz, 2002).

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human Stabilin-1.

Physical form

Format: Unpurified

Unpurified rabbit polyclonal in serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western blot in HUVEC lysate.
Western blot Analysis: 1:1,000 dilution of this antibody detected Stabilin-1 on 10 µg of HUVEC lysate.

Specificity

Other notable species homologies include mouse (87%) and rat (83%).

The antibody recognizes the C-terminus of Stabilin-1 within the cytoplasmic region of a cell.

Storage and Stability

Stable for 1 year at -20°C from date of receipt if stored in undiluted aliquots.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~ 275 kDa