Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 120 µg of fusion protein (35 kD). The stock solution of the antigen can be made up using 100 µL of PBS. For negative control, preincubate 3 µg of fusion protein with 1 µg of antibody for one hour at room temperature. For positive control, use 20 ng of fusion protein per lane. Optimal concentrations must be determined by the end user.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blot: 1:200-400 using ECL on HERG transfected HEK293 cells.

Immunocytochemistry

Immunoprecipitation

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Anti-Potassium Channel HERG Antibody, C-terminus is an antibody against Potassium Channel HERG for use in IC, IP & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: C-terminus

GST fusion protein corresponding to amino acids 1106-1159 of human erg1 (HERG) (Accession number Q12809).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Specificity

Recognizes HERG (hKv11.1, Voltage gated K+ channel subfamily H member 2, KCNH2, ether-a-go-go related channel). The antibody recognizes the HERG1b splice variant but not splice variants HERG1-3 or HERG-4. The immunogen shares similiarity with rat erg2 (22/54) and rat erg3 (21/54).

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Target description

127 kDa