Analysis Note

ControlHippocampal neurons, rat brain tissue lysates or mouse brain tissue lysate

Application

Detect Glutamate Receptor 1 using this Anti-Glutamate Receptor 1 Antibody, phosphoSer 831 validated for use in WB.

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Western blot and dot blots: 1:500-1:1000. The working dilution should be based on the tissue and the expected phosphorylation state.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to amino acids surrounding the phosphoSer831 of rat GluR1.

Epitope: phosphoSer 831

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 05-823

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.

Specificity

Recognizes Glutamate Receptor 1 [GluR1], phosphoSer831. The antibody recognizes a protein of ~100 kDa corresponding to GluR1 phosphorylated at Ser831 in rat hippocampal homogenate. Immunolabeling of the GluR1 protein is blocked by the immunogen peptide but not by the corresponding non-phosphopeptide. The immunogen sequence is 100% homologous in human and mouse.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

100 kDa