HEY 1/HRT1; RBX-100UG

Application

Western blot: 1:200-1:1,000 using ECL.

Immunohistochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Hey 1/HRT1 Antibody detects level of Hey 1/HRT1 & has been published & validated for use in WB, IH.

Research Sub CategoryDevelopmental NeuroscienceNeuronal & Glial Markers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide, amino acids 207-220 of human Hey 1 / HRT1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Specificity

Recognizes Hey 1 / HRT1, a Helix-loop-helix class of transcription factor.

Storage and Stability

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.


For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.