Analysis Note

ControlHuman neural stem cells, Raji cell lysate.

Application

Western Blot:
1:200-1:1,000 dilution using ECL.

Immunohistochemistry:
1:200-1:1,000 dilution of a previous lot worked.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental NeuroscienceNeuronal & Glial Markers

This Anti-HES-1 Antibody is validated for use in IH, WB for the detection of HES-1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Hes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.

Immunogen

Synthetic peptide.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Specificity

Reactivity with other species has not been confirmed.

Recognizes HES-1 (Hairy family), a Helix-loop-helix class of transcription factor.

Storage and Stability

Stable for 1 year at -80°C from date of receipt.

Target description

29 kDa