Application
Research Sub CategoryNuclear Receptors
This Anti-Sox9 Antibody, Alexa Fluor® 555 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.
Research CategoryEpigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sox9 in HepG2 cells.
The unconjugated version (Cat. No. AB5535) is also shown to be suitable for Western blotting and immunohistochemistry applications.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Transcription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences that encode for the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Sox9.
Legal Information
ALEXA FLUOR is a registered trademark of Life Technologies
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Affinity purified
Purified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox9 in HeLa cells.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
55-65 kDa observed
This product has met the following criteria to qualify for the following awards: