Analysis Note

ControlIncluded free of charge with the antibody is 40 µg of control antigen (lyophilized powder). For negative control, preincubate 1 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blotting: 1:200 using ECL on rat heart membranes. See suggested protocol.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.

Anti-Sodium Channel Nav1.5 Antibody, pain is an antibody against Sodium Channel Nav1.5 for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Highly purified peptide corresponding to residues 493-511 of rat Nav1.5 (Accession P15389).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 50 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Specificity

Recognizes Nav1.5 (Scn5a, SKM2). The epitope specific for Nav1.5 is not present in any other known proteins.

SPECIES REACTIVITIES:
The immunogen sequence is identical in mouse and conserved in human (17/19). Reactivity with other species has not yet been tested.

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months from date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.