Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Research CategoryNeuroscience

Western blot: 1:200-1:300 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Research Sub CategoryIon Channels & TransportersSignaling Neuroscience

This Anti-Calcium Channel Antibody, Voltage Gated β3 is validated for use in WB for the detection of Calcium Channel.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: Voltage Gated beta3

A purified peptide corresponding to amino acid residues 463-477 of rabbit Ca beta3 (Accession number P54286).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Format: Purified

Specificity

Recognizes the full length Ca beta3 protein, not with other known proteins tested so far.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.