GLYCINE RECPT; RB X-200UG

Application

Anti-Glycine Receptor Antibody detects level of Glycine Receptor & has been published & validated for use in IP & WB.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blot: 1:1000 using ECL

Immunoprecipitation: 1:10 following the protocol of Raymond et al., Nature (1993) 361:637-641.

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Peptide from the amino terminus of the human alpha1 glycine receptor subunit conjugated to KLH.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Lyophilized from ammonium bicarbonate (5mM) some residual salt may be present. Reconstitute with 100 µL of PBS. Contains no preservatives.

Specificity

Recognizes glycine receptor alpha1. Shows cross reactivity to glycine receptor alpha2. subunits The antibody has been used successfully in Western blot and immunoprecipitation assays with cell extracts containing recombinant human glycine receptors and with homogenates of mouse spinal cord. By Western blot the antibody recognizes a protein with a molecular weight of ~ 48 kD.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.