AB5038 Display Image

SYNUCLEIN; ALPHA; RB X-50UL

Code: AB5038 D2-231

Analysis Note

ControlHuman brain sections.

Application

Immunohistochemistry:
1:1,000-1:2,000 dilutoin of a previous lot was used on paraffi...


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Analysis Note

ControlHuman brain sections.

Application

Immunohistochemistry:
1:1,000-1:2,000 dilutoin of a previous lot was used on paraffin sections; 1:4,000-1:12,000 dilution of a previous lot was used on frozen sections.

Western blot:
1:1,000-1:2,000. Shows a 14-19kDa band.

Immunocytochemistry:
Previous lot used IC.

Optimal working dilutions must be determined by end user.

Anti-Synuclein Antibody, α detects level of Synuclein & has been published & validated for use in IC, IH, IH(P) & WB.

General description

alpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer′s Disease (AD). α-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including α-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).

Immunogen

Human alpha-synuclein residue 111-131 coupled to KLH.

Epitope: a.a. 111-131

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1053

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Rabbit polyclonal serum lyophilized, no preservatives. Reconstitute with 50 µL of sterile distilled water. Centrifuge to remove any residue.

Quality

Evaluated by Western Blot on Human Brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Synuclein on 10 µg of human brain lysates.

Specificity

Has not been tested on other species.

Alpha-synuclein. The immunizing peptide was chosen to reduce cross-reactivity of AB5038 with β-synuclein to a minimum. Immunohistochemical and Western blot analysis of human brain indicates a high level of specificity.

Target description

~14-19 kDa

antibody formserum
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... SNCA(6622)
manufacturer/tradenameChemicon®
NCBI accession no.NM_000345.2
Quality Level100
shipped indry ice
species reactivityrat, human
technique(s)immunocytochemistry: suitable, western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
UniProt accession no.P37840
This product has met the following criteria: