Analysis Note

ControlPOSITIVE CONTROL: NKp44 positive cells.

Application

Anti-DAP12 Antibody detects level of DAP12 & has been published & validated for use in IP.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Immunoprecipitation (2-4 µg/mg of protein lysate)

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells (Bakker, 2000, Campbell, 1999). DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction (McVicar, 1998). Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice (Bakker. 2000, Tomasello, 2000), as well as presenile dementia and bone cysts in humans (Paloneva, 2000).

Immunogen

Recombinant DAP12 protein.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in phosphate buffered saline with 0.08% sodium azide.

Format: Purified

Specificity

Recognizes DAP12 protein.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.