anti-phospho-Cortactin (Tyr466) (Mouse)

Application

This Anti-Cortactin Antibody, phospho-specific [Tyr466] is validated for use in WB for the detection of Cortactin.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cortactin is cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.

Immunogen

Phosphorylated cortactin (Tyr466) synthetic peptide corresponding to mouse amino acid residues around tyrosine 466 (tyrosine 470 in the human sequence).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antibody is cross-adsorbed to nonphospho-peptide corresponding to the site of phosphorylation and preadsorbed with phosphopeptides to cortactin tyrosine 421 and 482 to remove cross-reactivity then affinity purified using phosphorylated cortactin (Tyr466) peptide. Antibody is supplied in phosphate-buffered saline (no Ca²⁺, no Mg²⁺), pH 7.3, containing 1 mg/ml BSA, and 0.05% sodium azide.

Specificity

The antibody detects the 80-85 kDa protein of cortactin.

Storage and Stability

Maintain at -20°C for up to one year from date of receipt. Avoid repeated freeze/thaw cycles.