Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

This Anti-DEDAF Antibody is validated for use in WB for the detection of DEDAF.

Western blot: 0.5-1 µg/mL.

A549 or HepG2 cell lysate can be used as a positive control and a 32 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rabbit anti-DEDAF polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human-DEDAF. This sequence is identical to the mouse sequence.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified IgG by immunoaffinity chromatography. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Specificity

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. DEDAF (DED Associated Factor) is expressed in multiple tissues and cell lines. DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/Caspase-8 complexes at the cell membrane. DEDAF also interacts with DEDD (DED-containing DNA binding) protein in the nucleus, which indicates its involvement in the regulation of both cytoplasmic and nuclear events of apoptosis.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.