Application
Western blot: 1:500 to 1:1000
Human heart tissue lysate can be used as a positive control.
Optimal working dilutions must be determined by end user.
This Anti-Smac/Diablo Antibody is validated for use in WB for the detection of Smac/Diablo.
General description
The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
Immunogen
Peptide corresponding to aa 225-239 (EERAESEQEAYLRED) of human Smac/DIABLO.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Specificity
Detects Smac/DIABLO in an approximately 25 kDa band by western blot.
This product has met the following criteria to qualify for the following awards: