anti-phospho-PARP (214/215)

Analysis Note

ControlJurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).

Application

Research CategoryApoptosis & CancerMetabolism

For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.

Research Sub CategoryApoptosis - AdditionalEnzymes & Biochemistry

Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

Immunogen

Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-575; 04-576

Physical form

ImmunoAffinity Purified

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

Quality

Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.

Specificity

Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.

The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

Storage and Stability

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.