Application

Research CategoryMetabolism

Anti-Low Density Lipoprotein Antibody, copper oxidized is an antibody against Low Density Lipoprotein for use in ELISA, WB, IH.

Research Sub CategoryLipid Metabolism & Weight Regulation

Western Blot

Immunohistochemistry: 1:50-1:500

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: copper oxidized

Fully Cu²⁺-oxidized LDL.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum. Lyophilized. Contains no preservative. Reconstitute with 100 µL of sterile distilled water.

Specificity

Reacts strongly to fully oxidatized modifications of LDL including Cu2+-oxidized LDL, MDA-LDL, HOCL-LDL but not to other oxidized proteins like MDA-HSA, HOCL-HSA, MDA-HDL, HOCL-HDL (below detection limit). The reaction of native LDL was weak, but clearly detectable (approx. 20%). Minimally oxidized LDL gave a strong binding signal (>80%).

Storage and Stability

Maintain lyophilized material at 2-8°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.