Application

This Anti-Tie-1 Antibody, C-terminus is validated for use in ELISA, WB for the detection of Tie-1.

Research CategorySignaling

Western blot: 1-10 µg/mL using ECL. NIH/3T3 cells can be used as positive control. The predicted size of Tie-1 is ~135-140 kDa.

ELISA: (1:10,000 -1:100,000) using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: C-terminus

20 amino acid peptide sequence within the cytoplasmic, C-terminus of human Tie-1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA and 0.05% sodium azide.

Specificity

Recognizes human Tunica internal endothelial cell kinase (Tie-1), also described as tyrosine protein kinase receptor. Tie-1 (human 1138 aa; mouse 1134 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells. Tie-1 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2 Ig-like C2-type, and 3-EGF-like domains.

SPECIES REACTIVITIES: The peptide sequence used is 100% conserved in mouse, rat, and bovine. Antibody crossreactivity in other species is not established.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze and thaw.