Application

Western blot: 1-10 µg/mL using ECL. The predicted size of Ang-2 is ~55 kDa. Like Ang-1, its size may higher due to glycosylation.

ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well.

Optimal working dilutions must be determined by end user.

Detect Angiopoietin-2 using this Anti-Angiopoietin-2 Antibody, N-terminus validated for use in ELISA & WB.

Research CategoryCell Structure

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

20 amino acid peptide sequence near the amino-terminus of mouse Ang-2.

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin presented as liquid in PBS containing 0.1% BSA and 0.05% azide, pH 7.5

Specificity

Recognizes mouse Angiopoietin-2 (Ang-2). Mouse/human Ang-2 is a 496 AA secreted angiogenic protein (Suri et al. 1996). There is ~85% homology between mouse and human Ang-2. It is only 60% homologous with Ang-1 (Davis et al. 1996). Ang-2 may act as an antagonist of Ang-1 and Tie-2 (Suri et al. 1996). It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).

The immunogenic peptide sequence is 71% (15/21 aa) homologous with human Ang-2 and has no substantial homology with Ang-1. Antibody crossreactivity in other species is not established. The immunogen peptide is 85% conserved in porcine and 55% in chicken.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.