Anti-Bid (pS65) Polyclonal Ab

Analysis Note

ControlWestern Blot:: Jurkat cell lysate

Application

Anti-Phospho-Bid (Ser65) Antibody detects level of Phospho-Bid (Ser65) & has been published & validated for use in WB.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.

Immunogen

Epitope: Ser65

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified in PBS with 0.09% NaN3.

Antigen Affinity Purified

Quality

Evaluated by Western Blot on Jurkat cell lysate treated with (+/-) insulin.

Western Blot Analysis:
A 1:100~500 dilution of this antibody detected phospho-Bid-S65 on jurkat cell lysate treated with (+/-) insulin.

Specificity

Other species have not been tested.

The antibody reacts with the 22 kDa phospho-Bid-S65 protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

22 kDa