Anti-MMP-3; N-terminus

Analysis Note

ControlPMA-treated HL-60 concentrated media

Application

Immunohistochemistry Analysis: A 1:600 dilution from a previous lot detected MMP-3 in colorectal carcinoma and ductal carcinoma tissues.

Detect MMP-3 using this Anti-MMP-3 Antibody, N-terminus validated for use in WB, IH(P).

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MMP-3 (matrix metalloproteinase-3), also known as stromelysin-1, is a member of the matrix metalloproteinase (MMP) family of zinc endopeptidases. MMP's are required for removal of extracellular matrix machinery during embryo development, morphogenesis and tissue remodeling. Endogenous tissue inhibitors of metalloproteinases (TIMPs) are responsible for regulating MMP-3. Any disruption of this system can cause diseases such as arthritis, metastasis, atherosclerosis and tumor growth.

Immunogen

KLH-conjugated linear peptide corresponding to human N-terminal MMP-3.

Epitope: N-terminus

Linkage

Replaces: AB812

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in PMA-treated HL-60 concentrated media.

Western Blot Analysis: 0.1-1 µg/mL of this antibody detects MMP-3 in 10 µg of PMA-treated HL-60 concentrated media.

Specificity

Other homologies: Mouse and rat (64% sequence homology).

This antibody recognizes MMP-3 at the N-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~58 kDa observed.
An uncharacterized band may be seen at ~78 kDa