ANTI-NA+K+CL- Cotransporter 2

Analysis Note

ControlRat liver lysates

Application

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Anti-Na+K+Cl-Cotransporter 2 Antibody detects level of Na+K+Cl-Cotransporter 2 & has been published & validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle′s loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. SLC12A1 is kidney specific and is essential in the regulation of ionic balance and cell volume. It is a component of an electrically silent transporter system, mediating sodium and chloride reabsorption. Defects in SLC12A1 are the cause of Bartter syndrome type 1, an autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria.

Immunogen

Epitope: Cytoplasmic domain

KLH conjugate followed by NA+K+CL- Cotransporter 2 corresponding to the cytoplasmic domain.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Antigen Affinity Purified

Quality

Western Blot Analysis:
1:2,000 dilution of this lot detected NA+K+CL- Cotransporter 2 on 10 µg of rat liver lysate.

Specificity

This antibody recognizes the cytoplasmic domain of NA+K+CL- Cotransporter 2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa