Anti-Tbr1

Analysis Note

ControlHuman fetal brain tissue lysate

Application

Research CategoryNeuroscience

Anti-Tbr1 Antibody is an antibody against Tbr1 for use in WB, IH.

Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected Tbr1 in mouse frontal cortex tissue.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tbr1 gene is a member of a conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Tbr1, a putative transcription factor known to be expressed in post-mitotic neurons, has been found highly expressed in glutamatergic early-born cortical neurons. A similar protein has been disrupted in mice and shown to be critical for early cortical development, and causes loss of projection neurons in the olfactory bulbs and olfactory cortex. The C-terminal region the Tbr1 derived protein was found to be necessary and sufficient for association with the guanylate kinase domain of calcium/calmodulin-dependent serine protein kinase.

Immunogen

KLH-conjugated linear peptide corresponding to mouse Tbr1.

Epitope: Internal

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human fetal brain tissue lysate.

Western Blot Analysis: 0.025 µg/ml of this antibody detected Tbr1 on 10 µg of human fetal brain tissue lysate.

Specificity

This antibody recognizes Tbr1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 68 kDa is observed; however, UniProtKB/Swiss-Prot entry Q64336 (TBR1_MOUSE) lists a mass of 73.941 kDa